24662128
OBJECTIVE	Biopsy confirmed that cervical intraepithelial neoplasia ( CIN ) may naturally regress or progress .
OBJECTIVE	Currently , the risk assessment for CIN progression to cervical cancer is still not satisfactory in clinical practice .
OBJECTIVE	We investigated copy number and protein expression of TP63 and MYC and explored the possibility to use them as progression biomarkers .
METHODS	Copy numbers of TP63 and MYC , as well as human papilloma virus ( HPV ) integration status , were determined by fluorescence in situ hybridization in 39 patients with CIN and 66 patients with cervical cancer .
METHODS	Corresponding protein expressions were analyzed by immunohistochemistry .
METHODS	Receiver operating characteristic curves were used to measure the diagnostic test performance for the detection of cervical cancer from CIN .
METHODS	Sensitivity and specificity values of biomarkers were calculated .
RESULTS	The average copy number and expression of TP63 and MYC , as well as the HPV integration rate , increased in the progression of CIN to cervical cancer .
RESULTS	Receiver operating characteristic analysis for detection of cervical cancer resulted in area under the curve ( AUC ) values of TP63 copy number ( AUC , 0.96 ; 95 % confidence interval [ CI ] , 0.91-1 .00 ) , MYC copy number ( AUC , 0.92 ; 95 % CI , 0.85-0 .96 ) , TP63 expression ( AUC , 0.73 ; 95 % CI , 0.61-0 .85 ) , and HPV-16 integration ( AUC , 0.73 ; 95 % CI , 0.60-0 .85 ) .
RESULTS	MYC expression was not able to statistically distinguish cancer from CIN ( P = 0.393 ) .
RESULTS	The combinations increased the specificity slightly but not sensitivity .
RESULTS	Among them , TP63 amplification showed the best diagnostic performance .
CONCLUSIONS	Amplification and overexpression of TP63 and MYC , and HPV integration rate , are associated with the transition of CIN to cervical cancer .
CONCLUSIONS	Future studies on these biomarkers will help to assess the risk of CIN progression .

